LE Magnet Plate低洗脱磁力架

LE Magnet Plate

Low Elution Magnet Plate低洗脱磁力架 SKU: A000350

LE Magnet Plate quantity 

Used with 96-well PCR plates when low elution volumes are needed to ensure high product concentration. This plate allows you to elute in as little as 8-10 µl.

LE磁力架数量

用于96孔PCR板时，需要低洗脱量，以确保高的产物浓度。该板允许您在少至8-10 μ l洗脱。

When concentrated nucleic acids are required in downstream applications

Minimize reaction volume, maximize product concentration.

Elute in as little as 8 μL

下游应用中需要浓缩核酸时

最小化反应量，最大化产品浓度。

洗脱仅需8 μL

We are using this plate on our liquid handler for NGS purposes and works very well in our hands. We were able to lower volumes and thus reduce costs without loss of quality and repetitiveness.

So an absolute recommendation.

– Ewart de Bruijn

我们正在使用这个板在我们的液体处理程序为NGS的目的，在我们的手中工作得很好。我们能够降低产量，从而在不降低质量和重复性的情况下降低成本。

所以绝对推荐。

- Ewart de Bruijn

Maximize product concentration.

Unique magnet architecture for very low volume sample elution.

The LE magnet is on its way to being my favorite of the four different types I have to choose from in my lab. I have had no issues using it with single and multichannel pipettes down to as little as 7.5uL elution volumes. Separation times are super fast with low volumes (<20s, generally), and I don’t see any carry over.

Highly recommended.

– Peter

最大化产品浓度。

独特的磁铁结构，非常低体积的样品洗脱。

在我的实验室里，有四种不同类型的磁铁可供我选择，而LE磁铁正逐渐成为我最喜欢的一种。我在单通道和多通道移液器中使用它没有任何问题，低至7.5uL的洗脱量。分离时间非常快，体积很小(一般小于20秒)，而且我没有看到任何结转。

强烈推荐。

——彼得

How does the LE Magnet Plate work?

The magnets used in the LE Magnet Plate have a smaller hole diameter than the 96R and 96S magnet plates. PCR wells don’t sit as deeply into these magnets, causing the ring of beads to form lower in the well. This means less elution volume is required (approximately 8-10 µl) to fully cover the beads and elute the product.

LE磁力架是如何工作的?

LE磁力架中使用的磁铁具有比96R和96S磁铁板更小的孔直径。PCR孔并没有深入到这些磁铁中，导致珠环在孔的下部形成。这意味着需要更少的洗脱体积(约8-10 μ l)来完全覆盖珠子和洗脱产品。

Full Working Volume

Full Working Volume, Fast Separation Times, Low Elution Volume

全工作容积

充分的工作体积，快速分离时间，低洗脱体积

Tests with a working solution of Ampure® XP (1 part water + 1.8 parts Ampure) have shown the following settle times:

Alpaqua LE Bead Separation Times

Reaction Volume Separation Time

100 μL 4 minutes

200 μL 6-7 minutes

300 μL < 10 minutes

Ampure®XP(1份水+ 1.8份Ampure)工作溶液的测试表明:

Alpaqua LE珠子分离时间

反应体积分离时间

100 μ l 4分钟

200 μ l 6-7分钟

300 μ l < 10分钟

Important Consideration for Optimum Results

Built For Precision Automated Liquid Handling: The LE Magnet Plate is intended for use with automated liquid handling equipment. Use with manual pipettors is not recommended.  It performs best in conjunction with an advanced liquid handling instrument equipped with a multi-channel dispense head, such as the Beckman Biomek® liquid handlers or the Eppendorf epMotion® platforms.  As a general rule, if your robot can safely work with 384 well PCR plates, the LE Magnet Plate should work well.

Only Compatible With Flat PCR Plates: It is very important to only use flat PCR plates with the LE Magnet Plate. All wells must touch the LE magnets so the ring of magnetic beads will form at the correct position in the wells. Do not use warped PCR plates because if the wells do not touch the LE magnets, the magnetic beads will cluster at the bottom and cause bead carry-over in those wells.  The LE is not for use with single PCR strips.

对最佳结果的重要考虑

为精密自动化液体处理而建:LE磁铁板用于自动化液体处理设备。不建议与手动移液器一起使用。它与配备多通道分配头的先进液体处理仪器配合使用，如Beckman Biomek®液体处理器或Eppendorf epMotion®平台。一般来说，如果你的机器人可以安全地使用384孔PCR板，那么LE磁铁板应该工作得很好。

只兼容平板PCR板:只使用平板PCR板和LE磁板是非常重要的。所有的井都必须接触到LE磁铁，这样磁珠环就会在井中正确的位置形成。不要使用弯曲的PCR板，因为如果孔不接触LE磁铁，磁珠会聚集在底部，导致珠在这些孔中携带。LE不用于单个PCR条带。

Recommended PCR Microplates

The LE Magnet Plate works with most 96-well PCR plates – full-, semi-, or non-skirted.  The LE is not for use with single PCR strips.  Below are some common plates that we have confirmed reliably work.   Many others will work.  Please Contact Alpaqua if you would like to inquire about the compatibility of your specific PCR plate.

Eppendorf twin.tec 96 (full, semi, and non-skirted versions)

Abgene SuperPlate

Abgene AB-0800,  AB-1000 & Axygen PCR-96-FS-C

BioRad Hard-Shell Plate

推荐PCR微孔板

LE磁力架适用于大多数96孔PCR板-全，半，或非裙边。LE不用于单个PCR条带。下面是一些常见的板，我们已经确认可靠工作。其他许多方法也会奏效。如果您想查询您的特定PCR板的兼容性，请联系我们。

Eppendorf twin.tec 96(完整版、半版和无裙边版)

Abgene SuperPlate

Abgene AB-0800, AB-1000 & Axygen PCR-96-FS-C

BioRad硬壳板 

Not Recommended: ABI MicroAmp® Fast 96-Well Reaction Plate, 0.1 mL

不推荐:ABI MicroAmp®快速96孔反应板，0.1 mL